Formation and use of anaerobic consortia for the biotransformation of sulfur-containing extracts from pre-oxidized crude oil and oil fractions

Formation and use of anaerobic consortia for the biotransformation of sulfur-containing extracts from pre-oxidized crude oil and oil fractions

Crude oil

Methane

Biogas

Oxidation

Sulfur compounds

Gas condensates

Hybrid materials

Molybdenum compounds

Sodium compounds

Dimethylformamide

Immobilization

Anaerobic desulfurization

Artificial consortia

Bioluminescence

Polyvinyl alcohols

A new solution for fossil raw materials desulfurization based on a hybrid chemical-biocatalytic scheme with biogas and sulfide production is proposed.·H2O2, formic acid and Na2MoO4 were used for petroleum or oil fractions pre-oxidation. Ethanol or dimethylformamide was used as extractant to remove sulfur-contained compounds from pre-oxidized straight-run diesel oil fraction, non-hydro treated vacuum gas oil, gas condensate or crude oil. Compositions of cells (anaerobic sludge, Desulfovibrio vulgaris, Clostridium acetobutilycum, Rhodococcus ruber, Rhodococcus erythropolis) were specially developed, immobilized in poly(vinyl alcohol) cryogel and used for methanogenic treatment of sulfur-containing extracts, diluted with phosphate buffer (pH 7.2) and hydrolysates of renewable raw materials. The sulfur coming into the reactor with the extracts was 100% converted to inorganic sulfide or cell biomass. The ratio of methane in the biogas was 68–76%. Bioluminescent express-methods were used to control the possible toxicity of media and metabolic activity of cells used as biocatalysts.

319

Bioresource Technology

2021

2021-01-01

Maslova, Olga

Senko, Olga

Stepanov, Nikolay

Gladchenko, Marina

Gaydamaka, Sergey

Akopyan, Argam

Polikarpova, Polina

Lysenko, Sergey

Anisimov, Alexander

Efremenko, Elena

journalArticle

0960-8524

10.1016/j.biortech.2020.124248

https://www.sciencedirect.com/science/article/pii/S0960852420315224