High pyrolysis temperature biochar reduced the transport of petroleum degradation bacteria Corynebacterium variabile HRJ4 in porous media

High pyrolysis temperature biochar reduced the transport of petroleum degradation bacteria Corynebacterium variabile HRJ4 in porous media

Crude oil

Bacteria

Transport

Water

Pyrolysis

Gasoline

Biodegradation

Specific surface area

Mass spectrometry

Oxidation

Remediation

Petroleum transportation

Porous materials

Soil pollution

Oil spills

Mineral oils

Polycyclic aromatic hydrocarbons

Aromatization

Groundwater

Sodium chloride

Feedstocks

Equivalence classes

Organic carbon

Groundwater pollution

Negative ions

Phenols

Chemical bonds

Electrostatics

Gram-positive bacteria

HRJ4

Retention

Biochar has been widely applied for the remediation of petroleum-contaminated soil. However, the effect of biochar on the transport of petroleum degradation bacteria has not been studied. A typical Gram-positive petroleum degradation bacteria-Corynebacterium variabile HRJ4 was used to study the effect of different biochars on bacterial transport and retention. Results indicated that the addition of biochar in sand was effective for reducing the transport of bacteria and poplar sawdust biochar (PSBC) had a stronger hinder effect than corn straw biochar (CSBC). The hindrance was more evident with pyrolysis temperature of biochar raised from 300°C to 600°C, which was attributed to the increase of specific surface area (309 times). The hindrance effect also enhanced with higher application rate of biochar. Furthermore, the reduction of HRJ4 transport was more obvious in higher (25 mmol/L) concentration of NaCl solution owing to electrostatic attraction enhancement. The adsorption of biochar to HRJ4 was defined to contribute to the hindrance of HRJ4 transport mainly. Combining the influence of feedstocks and pyrolysis temperature on HRJ4 transport, it suggested that specific surface area had the greatest effect on HRJ4 transport, and pore-filling, electrostatic force also contributed to HRJ4 retained in quartz sand column. At last, phenol transportation experiment indicated that the restriction of biochar on HRJ4 enhanced the phenol removal rate in the column. This study provides a theoretical basis for the interaction of biochar and bacteria, which is vital for the remediation of oil-contaminated soil and groundwater in the field.

228-239

100

Journal of Environmental Sciences

2021

Guo, Saisai

Liu, Xiaomei

Zhao, Hang

Wang, Lan

Tang, Jingchun

journalArticle

1001-0742

10.1016/j.jes.2020.07.012

https://www.sciencedirect.com/science/article/pii/S1001074220303168