Bacterial community profile of the crude oil-contaminated saline soil in the Yellow River Delta Natural Reserve, China

Bacterial community profile of the crude oil-contaminated saline soil in the Yellow River Delta Natural Reserve, China

Crude oil

Bioremediation

Hydrocarbons

Biodegradation

Crude oil contamination

Contamination

Nitrogen

Proven reserves

Soil pollution

Soils

River pollution

Bacteriology

Aerobic bacteria

Dissolved oxygen

RNA

Biodiversity

16S rRNA

Bacterial community

Hydrocarbon degradation

Saline soil

Crude oil contamination greatly influence soil bacterial community. Proliferative microbes in the crude oil-contaminated soil are closely related to the living conditions. Oil wells in the Yellow River Delta Natural Reserve (YRDNR) region is an ideal site for investigating the bacterial community of crude oil-contaminated saline soil. In the present study, 18 soil samples were collected from the depths of 0–20 cm and 20–40 cm around the oil wells in the YRDNR. The bacterial community profile was analyzed through high-throughput sequencing to trace the oil-degrading aerobic and anaerobic bacteria. The results indicated that C15–C28 and C29–C38 were the main fractions of total petroleum hydrocarbon (TPH) in the sampled soil. These TPH fractions had a significant negative effect on bacterial biodiversity (Shannon, Simpson, and Chao1 indices), which led to the proliferation of hydrocarbon-degrading bacteria. A comprehensive analysis between the environmental factors and soil microbial community structure showed that Streptococcus, Bacillus, Sphingomonas, and Arthrobacter were the aerobic hydrocarbon-degrading bacteria

unidentified Rhodobacteraceae and Porticoccus were considered to be the possible facultative anaerobic bacteria with hydrocarbon biodegradation ability

Acidithiobacillus, SAR324 clade, and Nitrosarchaeum were predicted to be the anaerobic hydrocarbon-degrading bacteria in the sub-surface soil. Furthermore, large amount of carbon sources derived from TPH was found to cause depletion of bioavailable nitrogen in the soil. The bacteria associated with nitrogen transformation, such as Solirubrobacter, Candidatus Udaeobacter, Lysinibacillus, Bradyrhizobium, Sphingomonas, Mycobacterium, and Acidithiobacillus, were highly abundant

these bacteria may possess the ability to increase nitrogen availability in the crude oil-contaminated soil. The bacterial community functions were significantly different between the surface and the sub-surface soil, and the dissolved oxygen concentration in soil was considered to be potential influencing factor. Our results could provide useful information for the bioremediation of crude oil-contaminated saline soil.

133207

289

Chemosphere

2022

Gao, Yongchao

Yuan, Liyuan

Du, Jianhua

Wang, Hui

Yang, Xiaodong

Duan, Luchun

Zheng, Liwen

Bahar, Md Mezbaul

Zhao, Qingqing

Zhang, Wen

Liu, Yanju

Fu, Zhaoyang

Wang, Wei

Naidu, Ravi

journalArticle

0045-6535

10.1016/j.chemosphere.2021.133207

https://www.sciencedirect.com/science/article/pii/S004565352103681X